I just want to say that through this semester I have learned tremendously how to analyze data and think outside the box. I enjoyed this class tremendously since it allowed me to work at my own pace and explored things with help. I feel that I was not pressured in learning the materials in a set amount of time but can take it slow if I so choose. I enjoyed it greatly hope everyone a wonderful break.
I continued to look for mRNA reference sequence of other species. Ramsey helped me last week to find the correct one. There are a lot of provisional and model RAI1 from other species. I also found two articles about RAT1 that has to do with RAI1 in rat. I thought this was interesting but disappointing that I could not access the entire paper but could only read the abstract. Overall, the project is going well
A.)Begin SMS project
B.) I finally have a vague idea what to do on this project. I was planning on doing the proposed 1B –> looking at all the chromosomes of an individual or 3 –> drag tree of different species that have RAI1 relative to human
A.) Continue of Journal Club
B.) The journal club helps me explained the results and discussion. It helped cleared up a couple questions that are very confusing. I wish the paper had more information correlating with other papers.
A.) Journal discussion
B.) Through the journal club, I was able to understand the paper a little better. It was helped that Ramsey explained the process that was aCGH. Through the journal club, it helped clarified the methods section of the paper.
I finished the outlining the William et al. paper. I found this paper to be very interesting and the talked that Stephen given was very helpful in understanding the paper. I had a hard time with the methods and understanding some of the steps. I also find it difficult to keep track of all microdeletions/ microduplication and genes that were involved. Overall, I enjoyed the paper and looking forward to Journal Club today.
A.) We began the SMS project.
B.)
1. Stephen’s patients would fall in the microdeletion of 17p11.2.
2. The various diagnostic approaches to SMS are FISH, G-banding, MLPA, and qPCR. MLPA and qPCR can detect smaller deletions at higher resolution. MLPA
3. The proposed mechanism inducing SMS are microdeletions, including 17p11.2, are caussed by irregularities in the chromosomal recombination mechanism sponsored by repeat elements in the susceptible region of the genome.
4. RAI1 was determined as the main gene involved through the use of sequenced –> region of microdeletion.
5. The genotype-phenotype correlation described –> one of the phenotype is sleep disturbance –> especially for SMS.
6. The many phenotypes does not surprised me so much because usually many organ system are affect therefore, creating many phenotypes. The only phenotype that surprised me was that SMS patient would sleep all day due to the high dosage of melatonin.
I was relieved to finished my project. I felt that there are rooms for improvement. I feel that I should be able to explain it better. I feel that my explanation was not very clear. I should be working on that for the next presentation.
A.) Finished presentations
B.)
The presentations were a very successful in understanding more about HIV-1 env gene. I feel that each presentations supported and built upon one another in some respect. I enjoyed it a lot and learned a tremendously through the presentation. Through the presentation, it allowed me to see what other people used to analyze their data –> this may be useful for the future. I’m looking forward for the next paper.
A.) HIV Presentation
B). I really enjoyed the presentation today. I think that the presentations are allowing us to take a deeper look into the paper and try to understand it better. I feel that I have retained a lot of use information for future interaction with some of the tools. Through Sunny’s presentation, I learned more about Mega 4 and might be daring to attempt to use it. Hopefully everything will turn out ok. But overall, the presentations were great. Nice job guys!!!
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